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Samples were reduced with DTT and alkylated with iodoacetamide as described above, then diluted with DPBS (300 L), and taken forward to LysC and trypsin digestion without precipitation

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We immunoprecipitated OGDHc-E2 Lp glu adducts from HeLa cells treated with ML226, and then measured the levels of lipoate by immunoblot following short incubations of this immunoprecipitated OGDHc-E2 with purified ABHD11-Flag at 37 C

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